Fig. 4.

DEX facilitates the differentiation from naive CD4⁺T cells to Tregs in vitro. A Cell Counting kit-8 (CCK8) was used to detect the effect of DEX and Compound C on the proliferation of EL-4 cells. B Naive CD4⁺ T cells were extracted and purified from spleen. After cultured under Treg polarization for 72 h, the frequency of CD4⁺ CD25⁺ Foxp3⁺ Tregs in Controls, DEX, Compound C and DEX + Compound C groups was detected by flow cytometry. C The frequency of CD4⁺ Helios⁺ Foxp3⁺ Tregs in each group was detected by flow cytometry. D The expression of FOXP3 and IL-10 in EL-4 cells was detected by immunofluorescence. N = 5 in each group. All tests were repeated three times. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001