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. 2022 Sep 8;145(12):4349–4367. doi: 10.1093/brain/awac325

Figure 2.

Figure 2

Knockdown of KANSL1 affects pUb(Ser65) levels. (A) Quantification of pUb(Ser65) following treatment of SCR, PINK1 or NSL components siRNA KD POE SHSY5Y cells with 1 μM O/A for 1.5 h. Data are shown as mean ± SD; n = 6, one-way ANOVA with Dunnett’s correction. (B) Representative images of pUb(Ser65) (green) following treatment of SCR, PINK1 and KANSL1 KD POE SHSY5Y cells with 1 µM O/A for 3 h. Insets show the nuclei (blue) for the same fields. Scale bar = 20 μm. (C) Quantification of pUb(Ser65) in B (n = 3, two-way ANOVA with Dunnett’s correction). (D) Representative IB of mitochondrial fractions from SCR, PINK1 and KANSL1 KD POE SHSY5Y treated with 1 μM O/A for 1.5 or 3 h. (E) Quantification of pUb(Ser65) in D (n = 5, one-way ANOVA with Dunnett’s correction). (F) Quantification of PINK1 in D (n = 4, one-way ANOVA with Dunnett’s correction). Data are shown as mean ± SD.