Figure 3.
Ex vivo wire myography data showing relaxation responses of preconstricted thoracic aortic segments to increasing concentrations of methacholine (MCh) in offspring at 3 (A and B) and 7 mo (C and D) of age. Maternal exposure occurred with e-cigarettes with nicotine (ecig18 = 18 mg/mL) or without nicotine (ecig0 = 0 mg/mL). Controls are offspring with maternal exposure to ambient air. B and D: the maximal MCh dose (i.e., 10−5 M) from 3- and 7-mo-old offspring, respectively. All data are means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. controls. ecig, electronic cigarette.