Extended Data Figure 7. Identification and characterization of CB1 and CB2 hits from standard VLS of 115M Enamine REAL compounds.

(a) Chemical structures of the hits from the standard VLS. (b-c). Concentration-response curves of the best hits in β-arrestin recruitment Tango assays for antagonist activity at CB₁ (b) and CB₂ (c) receptors. The compounds rimonabant or SR144528 served as positive controls. The assays were carried out in the presence of 100 nM (EC₈₀) of the dual CB₁/CB₂ CP55,940 agonist. The data points are presented as mean ± SEM with n=3 independent experiments, each one carried out in triplicate. (d) Functional potencies and binding affinities of the hit compounds from standard VLS. The 95% Confidence Intervals (CI) were calculated from n=3 independent assays, with 16 dose-response points for functional K_i, values and 8 dose-response points for affinity Ki values, except for values marked with *, roughly estimated from 3-point assays.