Extended Data Figure 4. Concentration-response curves for V-SYNTHES hits in functional assays at CB₁ and CB₂ receptors (except those shown in main text Figure 3).

β-arrestin recruitment Tango assays were performed to assess antagonist activity of the compounds in (a-b CB₁ and (c-d) CB₂ receptors. The compounds rimonabant or SR144528 served as positive controls. The assays were carried out in the presence of 100 nM (EC₈₀ of the dual CB₁/CB₂ CP55,940 agonist. The data points are presented as mean ± SEM with n=3 independent experiments, each one carried out in triplicate.