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. 2022 Sep 22;27(12):4948–4958. doi: 10.1038/s41380-022-01778-2

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Distribution of leptin receptor (LepRb)-expressing cells (red) and oxytocin-immunoreactive neurons (green) in the different levels of PVN. Scale bars, 500 µm for low magnification and 100 µm for high magnification. b High-magnification image showing close apposition of puncta of LepRb-expressing neurons with oxytocin-positive neurons. Scale bar, 10 µm. c Oxytocin RNA expression levels 15 min after i.p. leptin injection. Left, hnRNA expression levels; right, mRNA expression levels. n = 8 male mice per group. **P < 0.01 compared with saline-treated group.