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. 2022 Apr 22;72(1):216–218. doi: 10.1136/gutjnl-2021-326617

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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(A) Representative TEM imaging of biliary organoids at 0 and 24 hours of SARS-CoV-2 virus infection. Coronaviruses were observed in the lumen of the organoid (arrows) and are found at the membrane-bound vesicles (arrows). Scale bar 500 nm to 5 µm. (B, C) Heatmaps depicting the 19 most significantly enriched genes related to viral infection and immune system on SARS-CoV-2 infection in liver and biliary organoids. Coloured bar represents the log2-transformed values. (D, E) KEGG orthology-based annotation system of differential gene expression profiles from SARS-CoV-2-infected liver and biliary organoids compared with mock infection. TEM, transmission electron microscope.