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. 2022 Dec 19;13(12):1053. doi: 10.1038/s41419-022-05500-6

Fig. 1. Irradiation induces apoptosis in OSCC cells.

Fig. 1

A MTS assay was used to determine the cell viability of CAL27/CAL27R and SCC25/SCC25R cells treated with/without IR (2 Gy) for 72 h. B Plate colony formation assay was performed to analyze the colony formation of CAL27/CAL27R and SCC25/SCC25R cells treated with/without IR (2 Gy) for 72 h. C Soft agar assay was employed to assess the anchorage-independent cell proliferation of CAL27/CAL27R and SCC25/SCC25R cells treated with/without IR (2 Gy) for 72 h. D Immunoblotting for γ-H2AX expression in CAL27/CAL27R and SCC25/SCC25R cells treated with/without IR (2 Gy) for 72 h. E z-VAD-fmk rescued IR-reduced cell viability. CAL27 and SCC25 cells were pretreated with z-VAD-fmk, Necrostatin-1, or 3-MA for 4 h, followed by IR (2 Gy) treatment for 72 h. F CAL27/CAL27R and SCC25/SCC25R cells were treated with/without IR (2 Gy) for 72 h. The cell lysates were prepared with the IP buffer. Cleaved-caspase 3 expression was determined by immunoblotting. All data are means ± s.e.m. *p < 0.05, ***p < 0.001, ns not statistically significant. a significant difference between groups as indicated.