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. 2022 Dec 19;13(12):1053. doi: 10.1038/s41419-022-05500-6

Fig. 2. TRAF4 is required for maintaining tumor cells radioresistance.

Fig. 2

A Immunoblotting for TRAF4 expression in CAL27/CAL27R and SCC25/SCC25R cells. B Immunoblotting for TRAF4 expression in TRAF4 knockout CAL27R and SCC25R cells. C MTS assay was used to determine the cell viability of TRAF4 knockout in CAL27R and SCC25R cells treated with/without IR (2 Gy) for 72 h. D, E Plate colony formation assay was performed to analyze the colony formation of TRAF4 knockout in CAL27R (D) and SCC25R (E) cells treated with/without IR (2 Gy) for 72 h. F, G Soft agar assay were employed to assess the anchorage-independent cell proliferation of TRAF4 knockout in CAL27R (F) and SCC25R (G) cells treated with/without IR (2 Gy) for 72 h. H The representative image of the Edu incorporation assay for TRAF4 knockout in CAL27R cells treated with/without IR (2 Gy) for 72 h. I TRAF4-null CAL27R and SCC25R cells were treated with/without IR (2 Gy) for 72 h. WCE was prepared with the RIPA buffer. γ-H2AX and cleaved-caspase 3 expressions were determined by immunoblotting. J Pretreated with z-VAD-fmk inhibitor for 4 h, MTS assay was used to determine the cell viability of TRAF4 knockout in CAL27R and SCC25R cells treated with/without IR (2 Gy) for 72 h. All data are means ± s.e.m. ***p < 0.001, a significant difference between groups as indicated.