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. 2022 Dec 19;13(12):1053. doi: 10.1038/s41419-022-05500-6

Fig. 6. TRAF4 knockout overcomes radioresistance in vivo.

Fig. 6

A–E TRAF4 knockout sensitizes OSCC cells to radiotherapy. Xenograft tumors derived from TRAF4 knockout CAL27R cells were treated with/without IR (2 Gy) for 25 days; n = 5 mice per group. Tumor growth curves (A), tumor mass images (B), and tumor weight (C) were recorded. Tumor sections were subjected to IHC staining of Ki67 and MCL-1 (D), and qualification analysis of Ki67 and MCL-1 protein expression (E). Scale bar, 25 μm. F–I JOSD1 introduction into TRAF4 knockout CAL27R cells rescues tumorigenesis under IR treatment. Xenograft tumors derived from TRAF4 knockout and ectopic expression JOSD1 CAL27R cells were treated with/without IR (2 Gy) for 27 days; n = 5 mice per group. Tumor growth curves (F), tumor mass images (G), and tumor weight (H) were recorded. Tumor sections were subjected to IHC staining of Ki67 and MCL-1 (I, left), and qualification analysis of Ki67 and MCL-1 protein expression (I, right). Scale bar, 25 μm. All data are means ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001, a significant difference between groups as indicated.