Table 1.
Example of calculation of relative fold enrichment
| Sample name | Target name | Reporter | Cт | # | Adjusted Cт to negative control | # # | Adjustment to input | Fold enrichment | Average fold enrichment |
|---|---|---|---|---|---|---|---|---|---|
| E2F6 IP | Zcwpw1-a | SYBR | 23.6 | 6.9 | 7.1 | 137.4 | 158.1 | ||
| E2F6 IP | Zcwpw1-a | SYBR | 23.3 | 7.2 | 7.5 | 178.8 | |||
| E2F6 IP | Zcwpw1-b | SYBR | 24.0 | 6.5 | 7.6 | 196.5 | 175.0 | ||
| E2F6 IP | Zcwpw1-b | SYBR | 24.4 | 6.1 | 7.3 | 153.6 | |||
| E2F6 IP | Rab8a | SYBR | 26.9 | 3.6 | 6.9 | 116.6 | 114.6 | ||
| E2F6 IP | Rab8a | SYBR | 27.0 | 3.5 | 6.8 | 112.6 | |||
| E2F6 IP | RNF130 | SYBR | 24.9 | 5.7 | 7.0 | 125.2 | 121.4 | ||
| E2F6 IP | RNF130 | SYBR | 24.9 | 5.6 | 6.9 | 117.6 | |||
| E2F6 IP | Neg control | SYBR | 30.9 | 30.5 | -0.4 | -0.4 | 0.8 | 1.0 | |
| E2F6 IP | Neg control | SYBR | 30.1 | 0.4 | 0.4 | 1.3 | |||
| Input | Zcwpw1-a | SYBR | 25.0 | -0.5 | -0.2 | ||||
| Input | Zcwpw1-a | SYBR | 24.5 | 0.0 | |||||
| Input | Zcwpw1-b | SYBR | 25.5 | -1.0 | -1.1 | ||||
| Input | Zcwpw1-b | SYBR | 25.8 | -1.3 | |||||
| Input | Rab8a | SYBR | 27.8 | -3.3 | -3.3 | ||||
| Input | Rab8a | SYBR | 27.8 | -3.3 | |||||
| Input | RNF130 | SYBR | 25.9 | -1.4 | -1.3 | ||||
| Input | RNF130 | SYBR | 25.7 | -1.2 | |||||
| Input | Neg control | SYBR | 24.6 | 24.5 | -0.1 | 0.0 | |||
| Input | Neg control | SYBR | 24.4 | 0.1 | |||||
| # Average of negative control | |||||||||
| # # Average of input | |||||||||