FIG. 3.

Nucleotide insertions and deletions identified in the sic gene of selected M1T1 isolates. The sic gene was completely sequenced in 20 selected isolates (10 from severe cases and 10 from nonsevere cases). Deletions (del) and insertions (ins) are with reference to the sequence of the sic allele deposited in the GenBank database (accession no. X92968). Among the severe isolates, one had a 30-bp deletion at 126, and another isolate had two deletions at 141 (121 bp) and 255 (12 bp). Among the nonsevere isolates, two isolates showed an 87-bp deletion at 456. All isolates showed an insertion of 9 bp at 645, and with the exception of two isolates from each group, all isolates showed an insertion of 15 bp at 141. A third insertion of 9 bp at 634 was seen in two isolates from the severe group and one from the nonsevere group.