TABLE 2.
Chemical composition of cell wall preparations useda
| Component | % Composition of arthritogenic strains
|
% Composition of nonarthritogenic L. fermentum ATCC 14931 | ||
|---|---|---|---|---|
| L. casei B ATCC 25303 | L. casei C ATCC 25302 | L. casei B ATCC 11578 | ||
| Carbohydrates | ||||
| Rhamnose | 5.2 | <0.1 | 15.2 | <0.1 |
| Glucose | 5.0 | 12.0 | 9.9 | 26.9 |
| Galactose | ND | ND | ND | 9.7 |
| Arabinose | ND | <0.1 | ND | ND |
| Fucose | ND | <0.1 | ND | <0.1 |
| N-Acetylmuramic acid | 1.3 | 4.0 | 5.3 | 6.0 |
| N-Acetylglucosamine | 4.3 | 5.4 | 8.2 | 8.1 |
| N-Acetylgalactosamine | 3.0 | 5.5 | 6.2 | ND |
| Amino acids | ||||
| Alanine | 4.3 | 2.9 | 1.7 | 4.5 |
| Glutamic acid/glutamine | 6.0 | 12.2 | 8.6 | 10.4 |
| Lysine | 3.7 | 7.0 | 4.8 | 1.9 |
| Aspartic acid/asparagine | 8.6 | 15.9 | 11.1 | 16.1 |
| Ornithine | 0.4 | 0.3 | 0.7 | 3.9 |
| Valine | 15.5 | 3.0 | 4.4 | ND |
| Glycine | 3.1 | 2.0 | 2.4 | 2.1 |
| Serine | 2.9 | 1.5 | 1.2 | 2.4 |
| Leucine | 8.2 | 2.9 | 2.6 | 9.2 |
| Isoleucine | 12.8 | 4.0 | 6.4 | 9.5 |
| Threonine | 5.0 | 1.9 | 3.8 | 3.8 |
| Proline | 1.6 | 1.1 | 1.6 | 0.7 |
| Tyrosine | 2.9 | 3.3 | 1.9 | 3.0 |
| α-Aminoisobutyric acid | 2.1 | 1.8 | ND | ND |
| Phenylalanine | 1.2 | 0.9 | 1.4 | 0.9 |
| Totalb | 96.7 | 87.3 | 97.4 | 118.8 |
a
Values are expressed as a percentage of cell wall (dry weight). Values of components of PG are in bold. ND, not detected.
b
Diaminopimelic acid, cystine, arginine, histidine, and cysteine were not detected.