TABLE 2.
Effect of saccharides on the binding of F1C fimbriated recombinant E. coli HB101(pPIL110-54) to immobilized asialo-GM1 (GgO4Cer) or asialo-GM2 (GgO3Cer)
| Saccharide | Inhibition (mean % of control)
|
||
|---|---|---|---|
| Conc (mM) | Asialo-GM1 (GgO4Cer) | Asialo-GM2 (GgO3Cer) | |
| d-Galactose | 10 | 53 | 57 |
| 1 | 12 | 10 | |
| Galβ1-3GalNAc | 10 | 51 | 37 |
| 1 | 8 | 6 | |
| Galβ1-4GlcNAc | 10 | 51 | 41 |
| 1 | 10 | 7 | |
| GalNAcβ1-4Gal-spacer-BSAa | 0.26 | 64 | 69 |
| 0.026 | 40 | 45 | |
| N-Acetyl-d-galactosamine | 10 | 47 | 47 |
| 1 | 12 | 9 | |
| Lactose | 10 | 48 | 41 |
| 1 | 11 | 8 | |
a
Molarity of disaccharide GalNAcβ1-4Gal linked to neoglycoprotein according to the company's information. Binding of nonfimbriated control HB101(pACYC 184) strain or fimbriated bacteria HB101(pPIL110-54) suspended in buffer only was used as a control value and was represented as 0.0 and 100%, respectively.