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. 2022 Aug 19;12(12):4472–4485. doi: 10.1016/j.apsb.2022.08.008

Figure 4.

Figure 4

(A) CLSM images of cellular mitochondrial state (Mitotracker) and nuclei (Hoechst) in 4T1 cells at different time points. Scale bar = 20 μm. (B) CLSM images of the mitochondrial membrane potential in 4T1 cells after different treatments indicated by JC-1 monomer (green) and aggregate (red). Scale bar = 10 μm. (C) Intracellular ATP content analysis. (D) Intracellular catalase activity analysis. Intracellular ROS generation in 4T1 cells after different treatments determined by (E) flow cytometry and (F) CLSM images. Scale bar = 20 μm. (G) Quantitative MFI values of intracellular ROS in 4T1 cells in different groups. Data are presented as the mean ± SD (n = 3). (H) Western blot assay for detecting the expressions of HSP-70 and HSP-90 in 4T1 cells after various treatments. 1: Control; 2: Control +40 °C; 3: CaCl2 + 40 °C; 4: CaS@PP; 5: I-CaS@PP; 6: I-CaS@PP + L; 7: I-CaS@PP + L + E. Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001. L means NIR laser irradiation, E means EGTA.