Figure 5.

(A) In vivo fluorescence images of 4T1 tumor-bearing mice after intravenous injection of free ICG and I-CaS@PP at different time points and (B) corresponding MFI values in the tumor site. Data are presented as mean ± SD (n = 3). ∗∗P < 0.01. (C) Ex vivo fluorescence images and (D) corresponding MFI values of major organs and tumor tissue harvested at 24 h postinjection. He: heart, Li: liver, Sp: spleen, Lu: lung, Ki: kidney, and Tu: tumor. Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001. (E) In vivo IR thermal images of 4T1 tumor-bearing mice in different groups under 808 nm laser irradiation (1.2 W/cm²) taken at different time intervals. (F) Corresponding temperature change in the tumor site with different treatments during laser irradiation. Data are presented as mean ± SD (n = 3). ∗∗∗P < 0.001. (G) Tumor growth curves after intravenous injection of saline, saline + L, ICG, ICG + L, CaS@PP, I-CaS@PP and I-CaS@PP + L. Data are presented as mean ± SD (n = 5). ∗∗P < 0.01, ∗∗∗P < 0.001. (H) Tumor tissues excised from euthanized 4T1 tumor-bearing mice after different treatments. (I) Individual tumor growth curves of mice in different groups. L means NIR laser irradiation.