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. 2022 May 13;12(12):4407–4423. doi: 10.1016/j.apsb.2022.05.009

Figure 8.

Figure 8

High expression of SERCA2 renders TNBC tumor preferentially sensitive to RL71 treatment. (A–F) Female nude mice were inoculated with SUM1315 cells stably expressing vectors or SERCA2 (SERCA2-OE) into the right flank, and administered with olive oil, RL71 (2 mg/kg/day) or PTX (10 mg/kg/7 days) 7 days later. The mice (n = 6/group) were sacrificed on Day 15 after administration. And tumors and lung tissues were collected, followed by photography and measurement. (A) The tumor growth curves. Left panel: representative images of the tumors (n = 6). (B) Tumor weight. (C) The proportion of mice with lung metastases in each group. Upper panel: representative images of lung section stained with H&E. Black arrowheads indicate metastatic foci in the lungs. Scale bar: 100 μm. (D) IHC for Ki-67 in tumor tissues. Lower panel: quantification of IHC for Ki-67. Scale bar: 100 μm. (E) IHC for p62 in tumor tissues. Lower panel: quantification of IHC for p62. Scale bar: 100 μm. (F) Western blot analysis of LC3B and p62 in tumor tissues. (G–I) Female nude mice were inoculated with MDA-MB-231 cells stably expressing SERCA2 into the mammary fat pad. After 14 days, the mice (n = 6/group) were treated as described above. (G) The tumor growth curves. (H) Tumor weight. (I) Western blot analysis of LC3B and p62 in tumor tissues. Animal experiments were performed twice independently. Data are shown as the mean ± SD of at least three independent experiments. #P < 0.05, ∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.001.