Figure 1.

Levels of NOX1 and NOX4 expression are upregulated in human breast cancer tissues, and radiation further increased NOX4 expression. Normal breast tissues were obtained from the adjacent normal tissue areas from surgically resected breast. Breast I, II, and VI tumors were triple negative breast cancer (TNBC), Breast VII (P), and (LM) were ER+ breast cancer tissues obtained from surgically resected primary (P) and lymph node metastases (LM). Breast IX was ER+ breast cancer. Frozen tissue sections were used for immunofluorescence labeling of anti-NOX1 and NOX4 antibodies. (A) Detection of the levels of NOX1 and NOX4 in breast normal and cancer tissues. The mean fluorescence intensity was quantified from 4 to 10 images using the NIH ImageJ. (B) Effect of radiation on NOX1 and NOX4 expression. Frozen tissue sections of a residual tumor of Breast VII PDX model following 10 Gy radiation were examined. Western blot used cell lysates from MCF-7 cells following 4 h of 5 Gy irradiation. The mean relative level of NOX4 over β-actin from three blots is shown.