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. Author manuscript; available in PMC: 2023 Nov 22.
Published in final edited form as: ACS Nano. 2022 Oct 18;16(11):18708–18728. doi: 10.1021/acsnano.2c07440

Figure 6.

Figure 6.

HANP/GKT831 treatment downregulated the expression levels of DNA repair genes and enhanced radiation-induced DNA damage. (A) Levels of γH2AX in Breast VII PDX tumors detected by immunofluorescence labeling. The mean fluorescence intensity of 5–10 images from each treatment is shown. (B) Western blot analysis of the levels of γH2AX protein in breast cancer MCF-7 cells treated with RT alone, 0.5 or 1 μM of HANP/GKT831, without or with 5 Gy RT. The mean relative level of γH2AX protein is shown as the ratio of intensity of γH2AX vs β-actin from four repeat blots. (C) (left) Levels of expression of DNA repair genes in MCF-7 cells after HANP/GKT831 treatment for 24 h and RT for 4 h. (right) RNA-seq analysis of Breast VII PDX tumors obtained 5 d after the last treatment. The levels of expression of DNA repair genes are shown as Log2 decrease or increase compared to the no-treatment control. The mean value obtained from two PDX tumors in each group is shown.