Skip to main content
. Author manuscript; available in PMC: 2023 Nov 22.
Published in final edited form as: ACS Nano. 2022 Oct 18;16(11):18708–18728. doi: 10.1021/acsnano.2c07440

Figure 7.

Figure 7.

Effect of HANP/GKT831 treatment on tumor stromal cells. Frozen tissue sections of Breast VII PDX tumors after different treatments were examined for the changes in tumor stromal fibroblasts and macrophages using immunofluorescence labeling. (A) FAP+ and α-SMA+ stromal fibroblasts. The mean of the total FAP+ or α-SMA+ fluorescent signal of four images per treatment is shown. (B) Tumor-associated macrophages. CD68 is a pan–macrophage biomarker. CD163+ is a biomarker for M2-like macrophages. The number of CD68+ or CD163+ macrophages in each fluorescence image was quantified to determine changes in the macrophage populations after treatment. The mean number of positive cells per image field under a 20× lens of 5–6 images are shown. * p < 0.05, **p < 0.01, *** p < 0.005.