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. 2022 Dec 20;11(12):12294. doi: 10.1002/jev2.12294

FIGURE 5.

FIGURE 5

TEX promote TGFβ‐dependent angiogenesis in vivo. Mice received subcutaneous injections of growth‐factor depleted Cultrex®. Each animal received 2 plugs (CTRL: 500 μl Cultrex® + 100 μl PBS; TEX: 500 μl Cultrex® + 50 μg TEX protein in 50 μl PBS). TEX derived either from SCCVII cells (TEX murine) or from UMSCC47 cells (TEX). Plugs were harvested after 7 days. (A) Representative photographs of harvested plugs and representative images of immunofluorescence staining for CD31 for the detection of vascular structures and CD68 for the detection of macrophages (green fluorescence); nuclei were counterstained with DAPI (blue fluorescence). (B) Representative images of immunofluorescence staining for CD31 or CD68 (green fluorescence) in combination with staining for pSmad2 (red fluorescence); nuclei were counterstained with DAPI (blue fluorescence). (C) Hemoglobin content in plugs. (D) Representative images of immunofluorescence staining for CD68 (green fluorescence) in combination with iNOS or ARG1 (red fluorescence) and DAPI (blue fluorescence). (E) Histology‐based quantification of macrophage infiltration into plugs. The following phenotypes were quantified: CD68 positive cells, CD68/iNOS double‐positive cells, and CD68/ARG1 double‐positive cells. Black scale bars = 1 cm. White scale bars = 100 μm. Data are presented as means ± SEM. ***p < 0.001 vs. CTRL; ## p < 0.01 vs. TEX; + p < 0.05 vs. TEX; & p < 0.01 vs. TEX.