TABLE 1.
Recombinant RII reciprocal antibody titers by ELISA and reciprocal ED50 blocking titers for EBA-175 erythrocyte bindinga
| Mouse no. | Reciprocal rRII ELISA Titer (OD 0.5)
|
ED50 Reciprocal EBA-175 Blocking Titerc
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RII (±)
|
RII (−)
|
RII (+)
|
RII (±)
|
RII (−)
|
RII (+)
|
|||||||
| Ind | Avg (SD) | Ind | Avg(SD) | Ind | Avg (SD) | Ind | Avg (SD) | Ind | Avg (SD) | Ind | Avg (SD) | |
| 1 | 110,495 | 114,838 | 87,552 | 94,824 | 334,340 | 169,378 | 36,000 | 36,000 | 11,000 | 16,666 | 48,000 | 35,280 |
| 2 | 60,059 | (53,407) | 129,591 | (31,760) | 83,978 | (112,565) | 17,000 | (13,441) | 20,000 | (4,932) | 24,000 | (9,844) |
| 3 | 187,930 | 67,331 | 116,520 | 46,000 | 19,000 | 34,000 | ||||||
| 4 | 100,870 | —c | 142,675 | 45,000 | —c | 35,000 | ||||||
a
RII (±), glycosylated and unglycosylated FVO rRII; RII (−), unglycosylated FVO rRII; RII (+), glycosylated FVO rRII. Ind, individual.
b
All data are compared to adjuvant control.
c
Mouse died due to unknown cause.