. 2022 Nov 28;87(24):16351–16367. doi: 10.1021/acs.joc.2c02019

Table 1. Optimization of the CuAAC Reaction between Azide-SL 8 and COUPY-Alkyne 12^a^,^b.

entry	solvent	CuSO₄	sodium ascorbate	temp.	yield
1	CH₃CN/H₂O (1:1)	10 equiv	20 equiv	RT	no desired product^a
2	CH₃CN/H₂O (2:1)	25 equiv	25 equiv	50 °C	only traces^a
3	^tBuOH/H₂O (4:1)	25 equiv	25 equiv	RT	30%^b
4	^tBuOH/H₂O (4:1)	25 equiv	25 equiv	50 °C	5% (degradation)^b

LC–MS observations.

Isolated yield.

Table 1. Optimization of the CuAAC Reaction between Azide-SL 8 and COUPY-Alkyne 12a,b.