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. 2022 Nov 15;11(12):4193–4209. doi: 10.1021/acssynbio.2c00498

Table 1. Plasmids Constructed and/or Used in This Study.

plasmid genotype and relevant characteristics reference
pSET152 aac3(IV), oriT, lacZα, ΦC31int, attP, MCS (26)
pSET152BB BioBricks-compatible vector pSET152; aac3(IV)R, oriT, ϕC31int, attP, MCS (24)
pENSV1 BioBricks-compatible vector; aadAR, blaR, oriT, ϕSV1int, attP (24)
pENTG1 BioBricks-compatible vector; vphR, blaR, oriT, ϕTG1int, attP (24)
pOSV808 BioBricks-compatible vector; hphR, oriT, VWBint, attP, amilCFP (32)
pSnogaori pKC505-based E. coliStreptomyces shuttle vector harboring 30.26 kb fragment of nogalamycin biosynthetic gene cluster; aac(3)IV, SCP2* ori, pBR322 ori, λ cos sites Accession No. OM832358,33
pSET154BB pSET152BB with strong 5′-tt-sbi-A terminator and 3′-fd phage terminator This study,8,9
pENSV3 pENSV1 with strong 5′- ECK120010818-term and 3′- ECK120029600-spy-term terminators This study,10
pENTG3 pENTG1 with strong 5′- L3S2P21-term and 3′- L3S3P41-term terminators This study,10
pSET-S1 wild-type snoa123 fused to the medium ermE*p promoter; cloned in pSET152BB vector This study
pSET-S2 wild-type snoa123 fused to the strong kasOp* promoter; cloned in pSET152BB vector This study
pSET-S3 codon-optimized snoa123 fused to the strong kasOp* promoter; cloned in pSET152BB vector This study
pSET-A1 wild-type aknBCDE2F fused to the medium ermE*p promoter; cloned in pSET152BB vector This study
pSET-A2 wild-type aknBCDE2F fused to the strong kasOp* promoter; cloned in pSET152BB vector This study
pSET-D1 wild-type dpsABCDG fused to the medium ermE*p promoter; cloned in pSET152BB vector This study
pSET-D2 wild-type dpsABCDG fused to the medium kasOp* promoter; cloned in pSET152BB vector This study
pSET-O2 wild-type oxyABCD fused to the strong kasOp* promoter; cloned in pSET152BB This study
pSET-S2S5 kasOp*-snoa123+kasOp*-snoaDEMB cloned in pSET152BB This study
pSET-D1S5 ermE*p-dpsABCDG+kasOp*-snoaDEMB cloned in pSET152BB This study
pSET-D2S5 kasOp*-dpsABCDG+kasOp*-snoaDEMB cloned in pSET152BB This study
pSET-A1S5 ermE*p-aknBCDE2F+kasOp*-snoaDEMB cloned in pSET152BB This study
pSET-A1S5 kasOp*-aknBCDE2F+kasOp*-snoaDEMB cloned in pSET152BB This study
pSET-D1D5 ermE*p-dpsABCDG+kasOp*-dpsEFYdnrG cloned in pSET152BB This study
pSET-D2D5 kasOp*-dpsABCDG+kasOp*-dpsEFYdnrG cloned in pSET152BB This study
pSET-A1D5 ermE*p-aknBCDE2F+kasOp*-dpsEFYdnrG cloned in pSET152BB This study
pSET-A2D5 kasOp* -aknBCDE2F+kasOp*-dpsEFYdnrG cloned in pSET152BB This study
pSET-D1A5 ermE*p-dpsABCDG+kasOp*-aknAE1WX cloned in pSET152BB This study
pSET-D2A5 kasOp*-dpsABCDG+kasOp*-aknAE1WX cloned in pSET152BB This study
pSET-A1A5 ermE*p-aknBCDE2F+kasOp*-aknAE1WX cloned in pSET152BB This study
pSET-A2A5 kasOp*-aknBCDE2F+kasOp*-aknAE1WX cloned in pSET152BB This study
pSV-S4 kasOp*-snoaDEM cloned in pENSV1 This study
pSV-S5 kasOp*-snoaDEMB cloned in pENSV1 This study
pSV-A4 kasOp*-aknAE1W cloned in pENSV1 This study
pSV-A5 kasOp*-aknAE1WX cloned in pENSV1 This study
pTG-S6 sp44-snoaLCF cloned in pENTG1 This study
pTG-S7 sp44-snoaC+kyc34+snoaF cloned in pENTG1 This study
pTG-A6 sp44-aknGHU cloned in pENTG1 This study
pTG-D6 sp44-dnrCDE cloned in pENTG1 This study
pEN10001 kasOp*-aknBCDE2F+kasOp*-aknAE1WX+sp44-aknGHU cloned into pSET154BB This study
pEN10002 kasOp*-aknBCDE2F+kasOp*-aknAE1WX+sp44-snoaC+kyc34+snoaF cloned into pSET154BB This study
pEN10003 kasOp*-snoa123+kasOp*-snoaDEMB+sp44-aknGHU cloned into pSET154BB This study
pEN10004 kasOp*-snoa123+kasOp*-snoaDEMB+sp44-snoaC+kyc34+snoaF This study
pRW10000 codon-optimized kasOp*-snoa123+kasOp*-aknAE1WX+sp44-snoaLCF This study