Table 3. Cytotoxic Activities of Compounds 1–13a.
| IC50 μM |
IC50 μM |
|||
|---|---|---|---|---|
| compounds | A549 | PC3 | MKL1 | MCC26 |
| Nogalamycinone (1) | 30.30 ± 2.30 | 18.60 ± 3.61 | 29.30 ± 10.30 | 36.50 ± 5.34 |
| Auramycinone (2) | 31.00 ± 0.96 | 13.90 ± 3.66 | 26.10 ± 1.16 | 30.40 ± 2.01 |
| 7-Deoxy-nogalamycinone (3) | >80 | >80 | >80 | >80 |
| 7-Deoxyauramycinone (4) | >80 | >80 | >80 | >80 |
| 9,10-seco-7-Deoxy-nogalamycinone (5) | >80 | >80 | >80 | >80 |
| 4-β-d-Glucosyl-nogalamycinone (6) | >80 | >80 | >80 | >80 |
| Aklavinone (7) | 17.80 ± 6.24 | 7.72 ± 0.57 | 22.70 ± 1.79 | 26.10 ± 0.66 |
| Nogalamycin A (9) | 0.056 ± 0.011 | 0.021 ± 0.002* | 0.062 ± 0.035* | 0.095 ± 0.044* |
| Nogalamycin KO (10) | >80 | >80 | >80 | >80 |
| Nogalamycin R (11) | 32.30 ± 4.09 | 28.30 ± 3.10 | 18.50 ± 2.64 | 35.60 ± 2.73 |
| 3′,4′-Demethoxy-nogalose-1-hydroxy-nogalamycinone (12) | 2.88 ± 0.23 | 2.88 ± 0.79 | 1.07 ± 0.011 | 3.50 ± 0.36 |
| Aclacinomycin T (13) | 3.62 ± 0.087 | 2.00 ± 0.15 | 2.35 ± 0.78 | 10.70 ± 4.32 |
a
Cytotoxicity IC50 values were obtained after 72 h incubation. Actinomycin D and H2O2 [A549 (non-small-cell lung), PC3 (prostate) human cancer cell lines, and Merkel cells MKL1 and MCC26] were used as a positive control at 20 μM and 1 mM concentration, respectively (0% viable cells, n = 3, but *(n = 6)).