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. Author manuscript; available in PMC: 2023 Jul 19.
Published in final edited form as: Cell Rep. 2022 Jul 19;40(3):111103. doi: 10.1016/j.celrep.2022.111103

Figure 6. Reconstructions of band 3-Ank1-accessory protein complexes by integrative 3D modeling suggest Ank1 compression links the membrane to the cytoskeleton.

Figure 6.

(A) An overview of the cytoskeletal network supporting the membrane of RBC. A pseudohexagonal network of spectrin heterotetramer underlies the membrane and is anchored to the membrane by the band 3-Ank1 complex. The tetramer is attached to actin on the other end through the association of band 4.1, actin, spectrin and other proteins. An actin polymer can interact with 6 spectrin tetramers through band 4.1 (Lux, 2016) (adapted with permission from (Goodman, 2020)).

(B) Glycolytic enzymes such as GAPDH and PGK1 are anchored to the band 3-Ank1 complex which can accommodate these enzymes while ANK1 adopts either open or closed conformations (see Zenodo repository (IMP_supplemt_figures) for more details).

(C) Ank1 in an open form in the band 3-Ank1 complex. Ank1, GAPDH, and PGK1 are colored.

(D) Roughly a third of observed intramolecular Ank1 crosslinks support it adopting a closed form in situ relative to the extended conformation observed for purified ankyrin (Wang et al., 2014), suggesting that Ank1 is capable of adopting either open or closed conformations. (see Zenodo repository (IMP_supplemt_figures) for more details).