FIG. 2.

The SA85-1.1-specific and epitope 1-specific responses are MHC-II restricted. Splenocytes were isolated from chronically infected mice and incubated with medium alone, SA85-1.1 or GST proteins (20 μg/ml), or peptide c (5 μg/ml). In addition, either anti-MHC-II I-A MAb or anti-MHC-II I-E MAb, at a final concentration of 150 μg/ml, was added to the cultures. Cells were stained for flow cytometry and analyzed as described in the legend to Fig. 1. These data are representative of two independent experiments.