FIG. 3.

The SA85-1.1-specific and epitope 1-specific response is detected by intracellular IFN-γ staining during the acute phase of T. cruzi infection. Splenocytes were isolated from a day 10 infected mouse, and incubated with medium alone or proteins (20 μg/ml) (a) or medium alone or peptides (5 μg/ml) (b). Cells were stained for flow cytometry and analyzed as described in the legend to Fig. 1. These data are representative of three independent experiments.