Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 19;12:204. doi: 10.1186/s13578-022-00935-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — PARKIN inhibited Ang II-induced cardiomyocyte hypertrophy. A, Immunoblotting results exhibiting the protein levels of PARKIN in cardiomyocytes treated with Ang II at the indicated time. B, Protein levels of PARKIN in cardiomyocytes infected with Parkin adenovirus or β-gal adenovirus. C, Protein levels of PARKIN in cardiomyocytes infected with PARKIN siRNA or PARKIN scramble. D − F, Overexpression of PARKIN suppressed Ang II-induced cardiomyocyte hypertrophy. Cardiomyocytes infected with Parkin adenovirus or β-gal adenovirus were exposed to Ang II. (D) Sarcomere organization stained with phalloidin-TRITC conjugate; bar = 20 µm. Blue represent nucleus. Red represent F-actin. (E) Cell surface was calculated. ** p < 0.01. *** p < 0.001. n = 3 experiments per group. (F) The mRNA levels of ANP and BNP were analyzed by qRT-PCR. The results were normalized to GAPDH. * p < 0.05. **p < 0.01. *** p < 0.001. n = 3 experiments per group. G − I, Knockdown of PARKIN induced hypertrophic responses. Cardiomyocytes were infected with PARKIN siRNA or PARKIN scramble. G Sarcomere organization stained with Phalloidin-TRITC conjugate; bar = 20 µm. Blue represent nucleus. Red represent F-actin. H Cell surface was calculated. * p < 0.05. n = 3 experiments per group. I The mRNA levels of ANP and BNP were analyzed by qRT-PCR. The results were normalized to GAPDH. * p < 0.05. n = 3 experiments per group