Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 20;41:354. doi: 10.1186/s13046-022-02569-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — KLF4 binds to the promoter of LAMA4. A Schematic illustration of pGL3-based reporter constructs used in luciferase assays to examine the transcriptional activity of LAMA4 named P1, P2 and P3. B P1, P2 and P3 together with the Renilla luciferase plasmid were transfected into 293 T cells with or without KLF4 expression. The Renilla luciferase construct was used to control for transfection efficiency, and dual luciferase activity was measured. C-D P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS and 143B cells with or without KLF4 knockdown for 24 h. Dual luciferase activity was measured. E P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS and 143B cells with or without LINC00629 knockdown for 24 h, and the cells were collected. Dual luciferase activity was measured. F P2 together with the Renilla luciferase plasmid was transfected into MNNG/HOS cells with or without KLF4 or LINC00629 knockdown for 12 h, and the cells were then treated with 3 μM TM for another 24 h. Dual luciferase activity was measured. G LINC00629 was overexpressed in MNNG/HOS cells with or without KLF4 knockdown, and P2 together with the Renilla luciferase plasmid was transfected into the cells for 24 h. Dual luciferase activity was measured. H Schematic illustration of the KLF4 wild-type binding site (BS) and the matching mutant (BSM) that was used in luciferase assays. I The wild-type promoter (BS) or the matching mutant (BSM) together with the Renilla luciferase plasmid was individually transfected into MNNG/HOS cells with or without KLF4 overexpression. Dual luciferase activity was measured. J The wild-type promoter (BS) or the matching mutant (BSM) together with the Renilla luciferase plasmid was individually transfected into MNNG/HOS cells with or without KLF4 knockdown. Dual luciferase activity was measured. K-L ChIP analysis showed the binding of KLF4 to the promoter of LAMA4 in MNNG/HOS cells with or without LINC00629 overexpression. Isotype-matched IgG was used as a negative control. Data in B, C, D, E, F, G, I, J were analysed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001