Fig. 8.

LINC00629 suppresses ER stress-induced apoptosis and facilitates osteosarcoma progression by activating the KLF4-LAMA4 axis. A KLF4 or LAMA4 was knocked down in 143B cells with or without LINC00629 overexpression. These cells were treated with 3 μM TM for 48 h, and cell apoptosis was detected by Western blot. GAPDH was used as a loading control. B-C KLF4 or LAMA4 was knocked down in MNNG/HOS and 143B cells with or without LINC00629 overexpression. The cells (3000 cells/well) were then tested for cell growth in the colony formation assay. Viable colonies after 1 week were counted and are shown (B). Data are depicted as bar graphs (C). D-E The migration of the indicated cells was detected by Transwell assays. Representative images of crystal violet-stained culture plates are shown (D). Data are depicted as bar graphs (E). F-G The shRNA KLF4 (KLF4 KD) or shRNA LAMA4 (LAMA4 KD) was used to knocked down in MNNG/HOS cells with or without LINC00629 overexpression. These cells (10⁶ cells per mouse) were injected subcutaneously into nude mice (n = 5). The volume (F) and weight (G) of the tumours were calculated and analysed. H-I The shRNA KLF4 (KLF4 KD) or shRNA LAMA4 (LAMA4 KD) was used to knocked down in MNNG/HOS cells with or without LINC00629 overexpression. These cells (10⁶ cells per mouse) were injected intravenously into nude mice (n = 5 per group). Representative images of HE staining are displayed (I). Each group of metastatic nodules was assessed (H). Data in C, E, F, G, H were analysed by Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001