Fig. 3. Measuring the flow distribution and WSS using μPIV (a) GFP signal from hiPSC-ECs in the lumens was captured using widefield microscopy. (b) Fluorescent beads were perfused and beads displacement was captured with 286 fps. (c) Luminal diameter was measured. (d) Vector fields were constructed using the beads displacements and the maximum velocity was determined at the Centre of the lumen. (e) WSS was calculated using the diameter and velocity profile. Error bars show 99% confidence interval of a vector field.
