Fig. 5. Confocal microscopy of TUBA1B-hiPSC-ECs under WSS (a) hiPSC-ECs cultured in static conditions for 72 hours. (b) hiPSC-ECs cultured in static conditions for 48 hours and 24 hours under 0.3 Pa WSS. Representative images of 7 individual lumens are shown with (i) TUBA1B-eGFP-ECs (green) co-stained for (ii) F-actin (orange) and (iii) merged image. Nuclei are visualized with DAPI (blue) in all images. Scale bars 30 μm.