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. Author manuscript; available in PMC: 2022 Dec 20.
Published in final edited form as: J Vis Exp. 2022 Feb 9;(180):10.3791/63225. doi: 10.3791/63225

Figure 2: Schematic to illustrate the isolation of embryonic hearts.

Figure 2:

(A) Fertilized embryos are removed from the breeding tank and placed in a Petri dish containing E3 water and maintained at 28 °C for up to 5 days. (B) At desired age, embryos are anesthetized in situ using tricaine and transferred into PB in 5 mL centrifuge tubes for dissociation. (C) Embryonic heart isolation apparatus consists of a 10 mL syringe attached to a 19 G needle mounted above the dissociation tube on a bench stand, allowing the hands to perform the trituration. (D) Image of the isolated heart (day 4) attached to a patch-clamp pipette.