. Author manuscript; available in PMC: 2022 Dec 20.

Published in final edited form as: J Vis Exp. 2022 Feb 9;(180):10.3791/63225. doi: 10.3791/63225

Table 2:

Solutions for isolation of zebrafish VSM cells.

S1 BUFFER	0.1% BSA (w/v), 145 mM NaCl, 4 mM KCl, 10 mM HEPES, 10 mM Glucose, 0.05 mM CaCl₂, 1 mM MgCl₂, adjusted to pH 7.4 using NaOH
S2 BUFFER	2 mL S1, 4 mg Papain, 2 mg DTT
S3 BUFFER	2 mL S1, 3 mg Collagenase Type H, 2 mg Trypsin Inhibitor, 1 mg Elastase