FIG 1.

Schematic diagram summarizing the different self-hybridization experiments performed in this work. L. major Fn cells were transfected to generate parental clones carrying different drug resistance markers that were used in sand fly coinfection for self-mating, selection and cloning of double-drug-resistant hybrids. Self-hybridization experiments involving 3 different sets of parental clones were performed, highlighted by red dashed boxes. Each parental clone was also used for single infection of sand flies, recovery from individual flies, and subsequent isolation of parental subclones in culture. In total, 45 selfing hybrid clones and 35 parental subclones were generated, of which 41 of the selfing hybrids, and all 35 of the subclones were analyzed by WGS along with their respective parental clones. L. major Sd (LmSd) was included in the initial crosses between FnSat × FnHyg to evaluate the impact of, including a heterologous strain on the selfing frequencies.