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FIG. 6.

FIG. 6

Light microscopic analysis of J774 cells infected with S. flexneri strains. Bacteria were left in contact with macrophages for 30 min and then treated with gentamicin-containing medium for another 50 min (A to C) or 2 h (D). Macrophages were stained with a modified Wright's stain after infection with 2457T (A), M4243A1 (B), or pWR700 (ΔipaH) (C and D). (E) J774 cells cultured in serum-free medium for 6 h showing apoptotic cells; (F) noninfected macrophages. Magnification, ×1,000.