FIG 6.

Broad-spectrum antiviral activity of compound 5s. (a) Huh-7.5 cells were infected with a renilla luciferase expressing human coronavirus (hCoV-229E)-Rluc reporter virus (38) in the presence of compounds at the indicated concentrations for 48 h. The cytotoxicity of these compounds was assessed by MTT assays of uninfected Huh-7.5 cells exposed to the compounds for 48 h. Values were normalized to the DMSO control and averages of three independent replicates ± SD are shown. (b) Huh-7.5_ACE2 cells were transduced with recombinant vesicular stomatitis virus (VSV)-based pseudoparticles encoding firefly luciferase and resynthesized compounds 3s (25 μM) and 5s (17 μM) or DMSO. After 4 h postransduction, medium was changed, and luciferase activity was determined after 16 to 18 h as a measure of residual transduction. (c) Huh-7.5 cells were seeded in 96-well plates and inoculated with lentiviral pseudoparticles harboring the G proteins of VSV (black bar) or rabies virus (RABV; gray bar and compound 5s (17 μM), or DMSO and inoculum was removed after 16 h. Firefly luciferase activity was determined after another 72 h. Values of three independent biological replicates were normalized to their DMSO control and means and SD are shown. EBOV, ebola virus.