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. 2022 Nov 8;204(12):e00349-22. doi: 10.1128/jb.00349-22

FIG 11.

FIG 11

The HbpA-S monomer protein carrying amino acid substitutions in the Hb-binding site showed reduced binding to Hb and Hb-Hp. Purified recombinant proteins (2 μg per lane) were separated by SDS-PAGE, and subsequent analysis was performed as described in the legend to Fig. 7A. A representative experiment is shown.