FIG 10.

Western blotting of recombinant BxpB and ExsFB isolated from E. coli and stored in PBS. (A) rBxpB incubated at 4°C (lane 1) and −80°C (lane 2) overnight was subjected to SDS-PAGE, transferred to immobilon filters, and probed sequentially with anti-6×His tag and anti-rBxpB antisera. (B) His-tagged BxpB and ExsFB were purified from E. coli and incubated in PBS at 4°C for 2 weeks. The samples were subjected to SDS-PAGE and blotted with anti-His tag antiserum. Positions of faint bands are denoted by an asterisk. (C) BxpB sample prepared by a different author in a different year, indicative of the reproducibility of this process.