FIG 4.

L. reuteri restores social interaction-induced synaptic transmission in the DA neurons of the lateral VTA in Shank3B^−/−-Rag2^−/− mice. (A) Schematic of the experimental design. (B) Representative traces of AMPAR and NMDAR currents (top) and AMPAR/NMDAR ratio (bottom) in dopaminergic neurons of the lateral VTA in baseline condition or following social interaction with a stranger mouse. (n = 5 to 9 mice per group; Control baseline versus Control + stranger interaction: q = 5.396, P = 0.0057; Control baseline versus Shank3B^−/−-Rag2^−/− baseline: q = 0.1656, P > 0.9999; Control baseline versus Shank3B^−/−-Rag2^−/− + L. reuteri + stranger interaction: q = 4.185, P = 0.0452; Control baseline versus Shank3B^−/−-Rag2^−/− + stranger interaction: q = 1.044, P = 0.9457; Control + stranger interaction versus Shank3B^−/−-Rag2^−/− baseline: q = 5.900 P = 0.0023; Control + stranger interaction versus Shank3B^−/−-Rag2^−/− + L. reuteri + stranger interaction: q = 1.110, P = 0.9329; Control stranger interaction versus Shank3B^−/−-Rag2^−/− + stranger interaction: q = 4.511, P = 0.0266; Shank3B^−/−-Rag2^−/− baseline versus Shank3B^−/−-Rag2^−/− + L. reuteri + stranger interaction: q = 4.585, P = 0.0235; Shank3B^−/−-Rag2^−/− baseline versus Shank3B^−/−-Rag2^−/− + stranger interaction: q = 1.268, P = 0.8957; Shank3B^−/−-Rag2^−/− + L. reuteri + stranger interaction versus Shank3B^−/−-Rag2^−/− + stranger interaction: q = 3.268, P = 0.1712 [one-way ANOVA with Tukey test, F_4,28 = 7.230, P = 0.0004]). *, P < 0.05; **, P < 0.01; ns, not significant. Bar graphs show means ± the SEM with individual data points.