TABLE 2.
CRISPRi identifies multistress response genes in rich medium
| Tü3298 IDa | Name | Symbol | Function |
|---|---|---|---|
| Tü3298_95 | rRNA methyltransferase family protein | cntL | Staphylopine biosynthesis |
| Tü3298_97 | Nickel ABC transporter, nickel/metallophore periplasmic binding protein | cntA | Trace metal import |
| Tü3298_1030 | Phosphate ABC transporter, permease protein PstA | pstA | Transport; nickel and phosphate import |
| Tü3298_149 | Transporter, betaine/carnitine/choline transporter family protein | —b | Transport; osmoprotectant |
| Tü3298_1053 | Cobalamin biosynthesis CobT VWA domain protein | —b | Inorganic ion transport and metabolism |
| Tü3298_1077 | Peptide-methionine (S)-S-oxide reductase | msrA | Protein damage repair |
| Tü3298_1108 | Exonuclease, DNA polymerase III, epsilon subunit family domain protein | —b | DNA damage response |
| Tü3298_795 | Excinuclease ABC subunit C | uvrC | DNA damage response |
| Tü3298_1138 | Sensor protein SrrB | srrB | Signal transduction and stress response |
| Tü3298_2206 | PTSc system mannose-specific EIIBCA component | manP | Carbohydrate metabolism |
| Tü3298_370 | 1-Phosphofructokinase | pfkB | Carbohydrate metabolism |
| Tü3298_797 | Succinate dehydrogenase/fumarate reductase, flavoprotein subunit | sdhA | Energy production and conservation; TCA cycle |
| Tü3298_1955 | Nitrate reductase, alpha subunit | —b | Energy production and conservation; nitrate reductase |
| Tü3298_1106 | Asparagine-tRNA ligase | asnS | Translation |
| Tü3298_1465 | RNA pseudouridylate synthase family protein | —b | Translation |
| Tü3298_1054 | Sigma-54 interaction domain protein | —b | Nitric oxide reductase |
| Tü3298_1133 | Elastin-binding protein EbpS | ebpS | Adhesion protein |
| Tü3298_698 | 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase | menD | Coenzyme metabolism |
| Tü3298_1167 | Transcription antitermination factor NusB | nusB d | Transcription |
| Tü3298_108 | Conserved hypothetical protein | —b | Unannotated |
| Tü3298_116 | C4-Dicarboxylate anaerobic carrier family protein | —b | Unannotated |
| Tü3298_2208 | yhgE/Pip C-terminal domain protein | —b | Unannotated |
| Tü3298_181 | kxYKxGKxW signal peptide domain protein | —b | Unannotated |
| Tü3298_778 | Conserved hypothetical protein | —b | Unannotated |
| Tü3298_2157 | Putative membrane protein | —b | Unannotated |
a
Gene was designated a multistress response gene if it was identified as a hit by multiple guides in at least 50% of the rich medium stress conditions.
b
— indicates no data.
c
PTS, phosphotransferase system.
d
Annotated as essential in S. aureus.