FIG 8.

The acidic GSL synthesis inhibitor aureobasidin A blocks C. albicans filamentation in a medium-dependent way. (A) The respective solid, hypha-inducing media were supplemented with the indicated concentrations of aureobasidin A (AbA) or phytosphingosine (Phy). WT SD log-phase cells were spotted on the solid medium and were incubated in either the absence (WT) or the presence of 5 ng/mL AbA (WT_AbA) in the preculture. Combined supplementation with AbA and Phy resulted in increased growth inhibition, especially for AbA-pretreated cells (WT_AbA). (B) C. albicans WT filamentation in liquid, hypha-inducing medium with the indicated supplementation of AbA and Phy after 240 min. (C) SD log-phase cells of the WT, incubated in either the absence (WT) or the presence of 5 ng/mL AbA (WT_AbA) in the preculture, were transferred to liquid, hypha-inducing medium. Filamentation was examined after 240 min, and pretreated cells displayed no hyperfilamentous phenotype. Filamentation score: 0, yeast; +, germ tubes and small hyphae; ++, moderate filamentation; +++, extensive filamentation. Bar, 100 μm.