Supplemental Figure S2.

A: Co-immunofluorescence staining of enhancer of Zeste homolog 2 (EZH2) and the biliary marker SRY-box transcription factor 9 (SOX9) in liver tissues from un-injected wild-type (WT) control. B: Co-immunofluorescence staining of EZH2 and the biliary marker SOX9 in liver tissues from Notch1 intercellular domain/AKT/Sleeping Beauty–injected mice. The EZH2 signal is detected in the cholangiocarcinoma cells but not in the non-tumorous liver parenchymal cells. Note the background autofluorescence in the normal liver region. Scale bars: 50 μm (A); 100 μm (B).