Supplemental Figure S1.

Removal of extracellular hyaluronan or hyaluronan synthase (HAS) 2 RNA interference (RNAi) has no effect on increased α-smooth muscle actin (α-SMA) expression in Has1/3-null skin fibroblasts. A: The effect of 4-methylumbelliferone (4-MU) or hyaluronidase treatment on α-SMA expression. Left panel: Representative Western blot analysis images of α-SMA and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from lysates of fibroblasts [wild type (WT) versus Has1/3 null] treated with 4-MU (1 mmol/L), Streptococcus dysgalactiae hyaluronidase (Hyal; 0.3 U/mL), or 0.1% dimethyl sulfoxide as vehicle control (Veh). Right panel: Densitometric analyses of the specific bands on Western blot analyses from four independent experiments (α-SMA normalized to GAPDH). B: The effect of Has2 RNAi on increased α-SMA expression in Has1/3-null fibroblasts. Left panel: Representative Western blot analysis images of α-SMA and GAPDH from lysates of fibroblasts (WT versus Has1/3 null) transfected with Has2 siRNA or nontargeted Scrambled siRNA. Right panel: Densitometric analyses of the specific bands on Western blot analyses from six independent experiments (α-SMA normalized to GAPDH). Data are presented as means ± SD (A and B). ∗P < 0.05.