Skip to main content
. 2022 Dec 6;13(6):e02835-22. doi: 10.1128/mbio.02835-22

TABLE 2.

Comparison between agar dilution-based phenotypic resistance and antimicrobial resistance mechanism according to AMR genetic determinants for 141 C. coli isolates

Antimicrobial Phenotypic profile No. (%) of isolates AMR determinant(s) detected No. with AMR gene present/absent Correlation ratea (%) Sensitivityb (%) Specificityc (%) PPVd (%) NPVe (%)
Ciprofloxacin and nalidixic acid Resistant 138 (97.87) gyrA T86I 137/1 99.29 99.28 100 100 75.00
Susceptible 3 (2.13) 0/3
Gentamicin Resistant 80 (56.74) aph(3′)-III and/or aac(6′)-aph(2′') and/or ant(6)-Ia 77/3 89.36 96.25 80.33 86.52 94.23
Susceptible 61 (43.26) 12/49
Streptomycin Resistant 99 (70.21) aph(3′)-III and/or aac(6′)-aph(2′') and/or ant(6)-Ia 83/16 84.40 83.84 85.71 93.26 69.23
Susceptible 42 (29.79) 6/36
Tetracycline Resistant 137 (97.16) tet(O) 115/22 84.40 83.94 100 100 16.67
Susceptible 4 (2.84) 0/4
Erythromycin Resistant 83 (58.87) 23S rRNA A2075G and/or erm(B) 79/4 96.45 95.18 98.28 98.75 93.44
Susceptible 58 (42.13) 1/57
Azithromycin Resistant 85 (60.28) 23S rRNA A2075G and/or erm(B) 79/6 95.04 92.94 98.21 98.75 90.16
Susceptible 56 (39.72) 1/55
Totalf Resistant 760 (77.00) 707/53 92.60 93.03 91.19 97.25 79.62
Susceptible 227 (23.00) 20/207
a

Calculated as the sum of true positives and true negatives divided by all tested isolates.

b

Calculated by dividing true positives by the sum of true positives and false positives.

c

Calculated by dividing true negatives by the sum of true negatives and false negatives.

d

Calculated by dividing true positives by the sum of true positives and false negatives.

e

Calculated by dividing true negatives by the sum of true negatives and false positives.

f

For gentamicin and streptomycin, aph(3′)-III and/or aac(6′)-aph(2′') and/or ant(6)-Ia was used for calculation. For erythromycin and azithromycin, 23S rRNA A2075G and/or erm(B) was used for calculation.