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Time course of Mn(II) oxidation in macrocolony biofilms of MOB-513 strains. Top view shows MOB-513-pEmpty, MOB-513-pdgcB, and MOB-513-ppdeA macrocolonies at distinct stages of growth, exhibiting (or not) Mn(II) oxidation phenotypes (brown color). Macrocolonies were set on Lept (−Mn) and Lept-Mn (+Mn) plates, incubated at 28°C and imaged daily for 5 days. Red scale bar, 5 mm.
