TABLE 2.
Plasmids and strains used in this study
| Strain or plasmid | Genotype | Antibiotic | Reference |
|---|---|---|---|
| Plasmids | |||
| pEmpty | pBBR1MCS-5 (with nptII promoter) | Gm | (38) |
| pdgcB | pBBR1MCS-5 derived plasmid with dgcB inserted downstream the nptII promoter | Gm | (21) |
| ppdeA | pBBR1MCS-5 derived plasmid with pdeA inserted downstream the nptII promoter | Gm | (22) |
| pPROBE-KT | repp BBR1promoterless gfp | Km | (40) |
| p7013p | pPROBE-KT derived plasmid with the mop7013 promoter inserted upstream the gfp gene | Km | This work |
| p7014p | pPROBE-KT derived plasmid with the mop7014 promoter inserted upstream the gfp gene | Km | This work |
| pWspR19 | P. aeruginosa PAO1 containing pBBR2-MCS5 with wspR19 | Gm | (23) |
| Strains | |||
| P. resinovorans strains | |||
| MOB-513 | Manganese oxidizer, wild type | Cm | (11) |
| MOB-513-pEmpty | MOB-513 containing pEmpty | Cm, Gm | This work |
| MOB-513-pdgcB | MOB-513 containing pdgcB | Cm, Gm | This work |
| MOB-513-ppdeA | MOB-513 containing ppdeA | Cm, Gm | This work |
| MOB-513-pdgcB-pPROBE-KT | MOB-513-pdgcB containing pPROBE-KT | Gm, Km | This work |
| MOB-513-pdgcB-p7013p | MOB-513-pdgcB containing p7013p | Gm, Km | This work |
| MOB-513-pdgcB-p7014p | MOB-513-pdgcB containing p7014p | Gm, Km | This work |
| MOB-513-pEmpty-pPROBE-KT | MOB-513-pEmpty containing pPROBE-KT | Gm, Km | This work |
| MOB-513-pEmpty-p7013p | MOB-513-pEmpty containing p7013p | Gm, Km | This work |
| MOB-513-pEmpty-p7014p | MOB-513-pEmpty containing p7014p | Gm, Km | This work |
| MOB-513-pilZp::Tn-pEmpty | MOB-513 with the ALMAR3 transposon inserted into pilZp containing pEmpty | Gm, Tc | This work |
| MOB-513-pilZp::Tn-pdgcB | MOB-513-pEmpty with the ALMAR3 transposon inserted into pilZp containing pdgcB | Gm, Tc | This work |
| P. aeruginosa strains | |||
| PAO1-pEmpty | PAO1 containing pBBR2-MCS5-Empty | Gm | (23) |
| PAO1-pWspR | PAO1 containing pWspR19 | Gm | (23) |