TABLE 3.
Quantification of BMnOx production, bacterial growth, and lyophilization survival ratios of MOB-513-pEmpty, MOB-513-pdgcB, and MOB-513-ppdeA static cultures grown in Lept and Lept-Mn and results of analysis of groundwater Mn(II) oxidation by bacteria-inoculated sands
| Quantification | MOB-513-pEmpty |
MOB-513-pdgcB |
MOB-513-ppdeA |
|||
|---|---|---|---|---|---|---|
| Lept | Lept-Mn | Lept | Lept-Mn | Lept | Lept-Mn | |
| Initial BMnOx in culture (μg/mL) | NDa | 0.44 ± 0.08 | ND | 10.45 ± 0.30 | ND | ND |
| Fresh culture (CFU/mL) | (2.50 ± 0.27) × 1010 | (2.25 ± 0.24) × 1010 | (6.50 ± 0.24) × 1010 | (2.00 ± 0.22) × 1011 | (1.65 ± 0.22) × 109 | (2.55 ± 0.22) × 109 |
| Lyophiles (CFU/mL) | (3.25 ± 0.34) × 107 | (3.60 ± 0.31) × 107 | (1.93 ± 0.20) × 108 | (2.33 ± 0.27) × 109 | (9.9 ± 0.25) × 105 | (1.79 ± 0.24) × 106 |
| SR (%) | 0.130 | 0.160 | 0.296 | 1.165 | 0.06 | 0.07 |
| Groundwater BMnOx (μg/mL) produced by: | ||||||
| Fresh cultures adhered to sands | 1.25 ± 0.10 | 6.72 ± 0.25 | 15.98 ± 0.33 | 23.92 ± 0.41 | ND | ND |
| Lyophiles adhered to sands | 1.28 ± 0.09 | 6.46 ± 0.31 | 17.90 ± 0.35 | 24.10 ± 0.42 | ND | ND |
ND, not detected.