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. 2022 Nov 30;7(6):e00595-22. doi: 10.1128/msystems.00595-22

TABLE 2.

Numbers and qualities of MAGs generated from metagenomes from all combinations of sequencing, assembly, and binning approachesa

Sequencing
platform(s)		 Assembly
type		 Binning
approach		 No. of
metagenomes		 Total no.
of MAGs		 Total no. of
high-quality
MAGs		 Avg MAG
quality		 No. of
Pelagibacter
MAGs		 Pelagibacter
MAG
quality
Illumina SPAdes MaxBin2 23 271 22 −94.5 1 −133.8
Illumina SPAdes Vamb 23 62 34 42.3 0 NA
PacBio hifiasm-meta MaxBin2 14 147 1 −59 1 −156
PacBio metaFlye MaxBin2 14 245 1 −51 3 0, 0, −142
PacBio hifiasm-meta Vamb 14 82 3 −128.4 3 0, 0, −52
PacBio metaFlye Vamb 14 39 2 −4.8 0 NA
Illumina + PacBio hybridSPAdes MaxBin2 14 160 7 −90.8 1 −102.8
Illumina + PacBio hybridSPAdes Vamb 14 37 18 40.3 0 NA
a

Both Illumina replicates were included in binning analyses where possible. Values for MAGs in the genus Pelagibacter are also included due to their ecological importance and the known challenges associated with binning genomes from this taxon. NA, not applicable.